Crispr Cas System in Plant Genome Editing a New Opportunity in Agriculture to Boost Crop Yield

Crispr Cas System in Plant Genome Editing a New Opportunity in Agriculture to Boost Crop Yield

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Clustered regularly interspaced short palindromic repeats CRISPR/Cas9 technology evolved from a type II bacterial immune system develop in 2013 This system employs RNA-guided nuclease, CRISPR associated (Cas9) to induce double-strand breaks.The Cas9-mediated breaks are repaired by cellular DNA repair mechanisms and mediate gene/genome modifications.The system has the ability to detect specific sequences of letters within the genetic code and to cut DNA at a specific point.

Simultaneously with other sequence-specific nucleases, CRISPR/ Cas9 have already breach the boundaries and made genetic engineering Rain Sets much more versatile, efficient and easy also it has been reported to have increased rice grain yield up to 25-30 %, and increased tomato fruits size, branching architecture, and overall plant shape.CRISPR/ Cas also mediated virus resistance in many agricultural crops.In this article, we reviewed the history of the CRISPR/Cas9 system invention and its genome-editing mechanism.

We also described the most recent innovation of Oil Filter the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement.Abbreviations: CRISPR, clustered regularly interspaced short palindromic repeats; Cas, CRISPR associated; crRNA, CRISPR RNA; tracrRNA, trans-activating crRNA; PAM, protospacer adjacent motif; sgRNA, single guide RNA; gRNA, guide RNA; ssODN, single-stranded DNA oligonucleotide; DSB, double-strand break; NHEJ, non-homologous end joining; HDR, homology directed repair, CRISPRi ,CRISPR interference.